Sperm viability in aquatic species is increasingly being evaluated by motility analysis via computer-assisted sperm analysis (CASA) following activation of sperm with manual dilution and mixing by hand. User variation can limit the speed and control over the activation process, preventing consistent motility analysis. This is further complicated by the short interval (i.e., less than 15 s) of burst motility in these species. The objectives of this study were to develop a staggered herringbone microfluidic mixer to: 1) activate small volumes of Danio pearl zebrafish (Danio albolineatus) sperm by rapid mixing with diluent, and 2) position sperm in a viewing chamber for motility evaluation using a standard CASA system. A herringbone micromixer was fabricated in polydimethylsiloxane (PDMS) to yield high quality smooth surfaces. Based on fluorescence microscopy, mixing efficiency exceeding 90% was achieved within 5 s for a range of flow rates (from 50 to 250 μL/h), with a correlation of mixing distances and mixing efficiency. For example, at the nominal flow rate of 100 μL/h, there was a significant difference in mixing efficiency between 3.5 mm (754%; meanSD) and 7 mm (922%; P=0.002). The PDMS micromixer, integrated with standard volumetric slides, demonstrated activation of fresh zebrafish sperm with reduced user variation, greater control, and without morphologic damage to sperm. Analysis of zebrafish sperm viability by CASA revealed a statistically higher motility rate for activation by micromixing (564%) than manual activation (457%; n=5, P=0.011). This micromixer represented a first step in streamlining methods for consistent, rapid assessment of sperm quality for zebrafish and other aquatic species. The capability to rapidly activate sperm and consistently measure motility with CASA using the PDMS micromixer described herein will improve studies of germplasm physiology and cryopreservation.
Mixer activate
At the moment you can activate/deactivate an individual Insert effect by Alt + CLicking the bypass button. This is not available on the Insert header to allow Activation/Deactivation of ALL inserts on that channel. The Bypass option is available on the Insert Header in the Mixer, the Activate/Deactivate Effect is not.
For anyone who has Windows 11 Insider Preview Build 25281 installed, there is a new Volume Mixer. While Microsoft has not made it easy to activate the feature, it is possible. Here's what you need to know.
The instructions listed below will successfully Activate the Fat Channel Plugin Collection on a StudioLive Series III Console or Rackmount mixer. As a good first step, make sure that you have installed the plugins on your StudioLive Series III mixer with these instructions below:StudioLive Series III - Fat Channel Plugin InstallationFor instructions with Studio One 3 Installation and Activation of Fat Channel plugins, please see the link below:
NOTE: If you are installing these Plugins on multiple mixers, make sure to complete both Installation and Activation of the plugins with one mixer at a time, making sure the network/USB is disconnected or the mixer(s) are turned off before continuing.
If your mixer isn't turning on, there could be several issues with it. First, check the circuit breaker in your home to make sure power is supplied to the mixer. Unplug your mixer and wait 15 seconds to plug it back in. If this does not work, there could be an issue with your phase board. See the guide on how to replace the KitchenAid mixer phase board.
My mixer was squealing so I disassembled it. It was working fine other than making noise. I found metal shavings and a loose screw. I cleaned shavings, tightened the loose screw, lubricated and reassembled. Figuring this may be the fix to the noise. I plugged back in, turned it on and nothing. Power from outlet in working. When I turn on, it doesn't make any noise at all, like it's not getting power. I looked at electrical area and all connections seem fine.
Abstract. The number concentration of activated CCN (Na) is the most fundamental microphysical property of a convective cloud. It determines the rate of droplet growth with cloud depth and conversion into precipitation-sized particles and affects the radiative properties of the clouds. However, measuring Na is not always possible, even in the cores of the convective clouds, because entrainment of sub-saturated ambient air deeper into the cloud lowers the concentrations by dilution and may cause partial or total droplet evaporation, depending on whether the mixing is homogeneous or extreme inhomogeneous, respectively. Here we describe a methodology to derive Na based on the rate of cloud droplet effective radius (Re) growth with cloud depth and with respect to the cloud mixing with the entrained ambient air. We use the slope of the tight linear relationship between the adiabatic liquid water mixing ratio and Re3 (or Rv3) to derive an upper limit for Na assuming extreme inhomogeneous mixing. Then we tune Na down to find the theoretical relative humidity that the entrained ambient air would have for each horizontal cloud penetration, in case of homogeneous mixing. This allows us to evaluate both the entrainment and mixing process in the vertical dimension in addition to getting a better estimation for Na. We found that the derived Na from the entire profile data is highly correlated with the independent CCN measurements from below cloud base. Moreover, it was found that mixing of sub-saturated ambient air into the cloud at scales of 100 m and above is inclined towards the extreme inhomogeneous limit, i.e. that the time scale of droplet evaporation is significantly smaller than that for turbulent mixing. This means that ambient air that entrains the cloud is pre-moistened by total evaporation of cloud droplets before it mixes deeper into the clouds where it can hardly change the droplet size distribution, hence Re remains close to its adiabatic value at any given cloud depth. However, the tendency towards the extreme inhomogeneous mixing appeared to slightly decrease with altitude, possibly due to enhanced turbulence and larger cloud drops aloft. Quantifying these effects, based on more examples from other projects and high resolution cloud models is essential for improving our understanding of the interactions between the cloud and its environment. These interactions may play an important role in cloud dynamics and microphysics, by affecting cloud depth and droplet size spectra, for example, and may therefore influence the cloud precipitation formation processes.
When I use alsamixer from terminal, I see the audio interface, but when I hit F6 and select it, I get a message that there is no mixer for it. Obviously, the drivers for this functionality do not load or are simply not there.
The Reliant Pond/Lagoon Mixer continuously, andslowly, moves water along the bottom of a lagoon/pond, continually mixing theentire water column using a minimum of energy. When compared to standardsurface aerators or directional mixers, the is moreefficient, less costly to operate, and requires minimal maintenance.
The matrix mixer allows to mix any of the 4 input channels to output busses 1 to 3 with a gain variation of -20dB to +20, optional phase invert.Clicking the gain knob cycles between "add" (gray knob), "off (-inf dB)" (black, flat) and "subtract (invert polarity)" (red knob) states.
Install the alsa-utils package. This contains (among other utilities) the alsamixer(1) and amixer(1) utilities. amixer is a shell command to change audio settings, while alsamixer provides a more intuitive ncurses based interface for audio device configuration.
Putting the previous example regarding defaults.pcm.card and defaults.pcm.device into practice, assuming we have 2 cards with index 0 and 1 respectively and wish to simply change the default card to index 1, would lead to the following configuration in /etc/asound.conf or the user-specific /.asoundrc to change both the playback and the mixer control card.
The plugin automatically allows multiple sources to play through it without problems so setting is as a default is actually a safe choice.If this is not working, you have to setup your own dmixer for the upmixing PCM like this:
The volume mixer was previously removed from the Quick Access menu by Microsoft. however, they are now preparing to restore it with a new design. Learn how to activate it today using the given guide.The Volume Mixer is a Windows application that allows you to control the output volumes for individual apps and programs. You can also manage the output device on your computer for different applications.
That said, at the time of writing this post, this feature is still experimental and is therefore hidden. However, you can enable the volume mixer in the Quick Access menu using ViVeTool.Let us show you how to enable the new volume mixer in Windows 11 with all the legacy mixer options.Note: At the moment, this method will only work for the Windows Insiders having Windows 11 Build 25281 or higher.
Follow the steps given below to activate the volume mixer directly from the Quick Access menu in the taskbar:Open GitHub and download the ViveTool Zip command line version.Download ViVeToolif(typeof ez_ad_units!='undefined')ez_ad_units.push([[300,250],'itechtics_com-box-4','ezslot_7',187,'0','0']);__ez_fad_position('div-gpt-ad-itechtics_com-box-4-0');Extract the contents of the compressed, downloaded ZIP file.Extract ViVeToolLaunch Command Prompt with elevated privileges.
The new volume mixer in Windows 11 is still an experimental feature by Microsoft. If you wish to try it out today, you will need to subscribe to the Dev channel and then use ViVeTool to activate the feature.However, we believe that this feature will eventually make its way to the stable channel as it was much needed in Windows 11.Let us know what you think about the new mixer design in the comments below. 2ff7e9595c
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